Vitamin D receptor is not essential for extracellular signal-related kinase phosphorylation by vitamin D(3) in human Caco-2/TC7 cells

Jun Yamauchi; Mariko Sekiguchi; Tomomi Shirai; Yoshiko Ishimi

doi:10.1271/bbb.120298

Vitamin D receptor is not essential for extracellular signal-related kinase phosphorylation by vitamin D(3) in human Caco-2/TC7 cells

Biosci Biotechnol Biochem. 2012;76(8):1588-90. doi: 10.1271/bbb.120298. Epub 2012 Aug 7.

Authors

Jun Yamauchi¹, Mariko Sekiguchi, Tomomi Shirai, Yoshiko Ishimi

Affiliation

¹ Section of Food and Nutrition Labeling, Department of Food Function and Labeling, National Institute of Health and Nutrition, 1-23-1 Toyama, Tokyo 162-8636, Japan. junyama@nih.go.jp

PMID: 22878203
DOI: 10.1271/bbb.120298

Abstract

Vitamin D(3) initiated rapid extracellular signal-related kinase (ERK) phosphorylation, but the contribution of vitamin D receptor (VDR) to this event is unclear. We investigated the use of RNA interference (RNAi) to knockdown VDR. RNAi downregulated VDR as well as its targeted gene expression, but vitamin D(3) dependent ERK phosphorylation remained. Thus VDR might not be involved in ERK phosphorylation by vitamin D(3).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Caco-2 Cells
Cholecalciferol / metabolism
Cholecalciferol / pharmacology
Gene Expression Regulation* / drug effects
Humans
Mitogen-Activated Protein Kinase 1 / genetics
Mitogen-Activated Protein Kinase 1 / metabolism*
Mitogen-Activated Protein Kinase 3 / genetics
Mitogen-Activated Protein Kinase 3 / metabolism*
Phosphorylation / drug effects
RNA, Small Interfering / genetics
Receptors, Calcitriol / antagonists & inhibitors
Receptors, Calcitriol / genetics*
Signal Transduction / drug effects
Signal Transduction / genetics

Substances

RNA, Small Interfering
Receptors, Calcitriol
Cholecalciferol
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3