Objective: To establish the analytical method for the fingerprint of Eucommiae Cortex by HPCE and compare the fingerprints of Eucommiae Cortex and its processed products.
Methods: Based on the mode of high performance capillary electrophoresis, 60 mmol/L sodium borate-20 mmol/L sodium dihydrogen phosphate-10% methanol (pH 10.0) was used as buffer solution. The separation voltage was 20 kV and the detection wavelength was set at 210 nm. Pinoresinol diglucoside was used as a reference standard, the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation softwarewas used to compare the similarity of samples.
Results: HPCE fingerprints with 10 common peaks of Eucommiae Cortex were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Eucommiae Cortex and its processed products, there were obvious differences in the relative areas of common peaks.
Conclusion: The method is reliable, accurate and can be used for quality control of Eucommiae Cortex.