Study on preparation of universal plasma in Chinese Han population

Abstract

Background: Universal plasma proves its importance in emergency situations where it simplifies the logistics by eliminating the need for ABO-match. There are now two available products of ABO-universal plasma internationally. Such product is not available in China yet. Besides the titers of anti-A and anti-B antibodies were different among ethnic populations for the same blood type, the distribution of blood types was also different. To improve the current status of plasma usage, this study was carried to explore optimal preparation procedures of universal plasma from Chinese Han population.

Methods: The optimal plasma ratios of A:B, A:AB and B:AB were evaluated by saline agglutination and the optimal ratio of A:B:AB was established. Three factors influencing the preparation of universal plasma including the proportion of plasma, reaction temperature and time were analyzed by an orthogonal design.

Results: The optimal plasma ratios between the two kinds of plasma were A:B=1:3, B:AB=1:3 and A:AB=1:2, respectively. Orthogonal design showed that the preparation of universal plasma in Chinese Han population could be carried by pooling plasma with the ratio of A:B:AB=6:2.5:1.5 at 22°C within 1h.

Conclusion: We have found an optimal ratio of pooled plasma for the preparation of universal plasma from Chinese Han population. Our study might provide preliminary evidence that Chinese Han donor plasma could contribute to a Uniplas product being Asian populations and world-wide applicable. The Uniplas anti A and B titers of (below) 1:2 is even lower than currently accepted.