Synthesis of homo- and heterofunctionalized glycoclusters and binding to Pseudomonas aeruginosa lectins PA-IL and PA-IIL

J Org Chem. 2012 Sep 7;77(17):7620-6. doi: 10.1021/jo300826u. Epub 2012 Aug 21.

Abstract

Homo- and heterofunctionalized glycoclusters with galactose and/or fucose residues targeting both PA-IL and PA-IIL lectins of Pseudomonas aeruginosa were synthesized using "Click" chemistry and DNA chemistry. Their binding to lectins (separately or in a mixture) was studied using a DNA Directed Immobilization carbohydrate microarray. Homoglycoclusters bind selectively to their lectin while the heteroglycocluster binds simultaneously both lectins with a slight lower affinity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Click Chemistry
  • Fucose / chemical synthesis
  • Fucose / chemistry*
  • Galactose / chemical synthesis
  • Galactose / chemistry*
  • Lectins / chemical synthesis
  • Lectins / chemistry*
  • Molecular Structure
  • Pseudomonas aeruginosa / chemistry*

Substances

  • Lectins
  • Fucose
  • Galactose