Iterative operations on microdroplets and continuous monitoring of processes within them; determination of solubility diagrams of proteins

Monika E Dolega; Slawomir Jakiela; Michal Razew; Agata Rakszewska; Olgierd Cybulski; Piotr Garstecki

doi:10.1039/c2lc40174f

Iterative operations on microdroplets and continuous monitoring of processes within them; determination of solubility diagrams of proteins

Lab Chip. 2012 Oct 21;12(20):4022-5. doi: 10.1039/c2lc40174f.

Authors

Monika E Dolega¹, Slawomir Jakiela, Michal Razew, Agata Rakszewska, Olgierd Cybulski, Piotr Garstecki

Affiliation

¹ Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.

PMID: 22868285
DOI: 10.1039/c2lc40174f

Abstract

We demonstrate a technique for controlling the content of multiple microdroplets in time. We use this system to rapidly and quantiatively determine the solubility diagrams of two model proteins (lysozyme and ribonuclease A).

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Microfluidic Analytical Techniques / instrumentation
Microfluidic Analytical Techniques / methods*
Muramidase / chemistry*
Ribonuclease, Pancreatic / chemistry*
Solubility

Substances

Ribonuclease, Pancreatic
Muramidase