Tomographic imaging of ratiometric fluorescence resonance energy transfer in scattering media

Appl Opt. 2012 Jul 20;51(21):5044-50. doi: 10.1364/AO.51.005044.

Abstract

A method to visualize and quantify fluorescence resonance energy transfer (FRET) in scattering media is proposed. It combines the ratiometric FRET method with fluorescence molecular tomography (FMT) in continuous wave (CW) mode. To evaluate the performance of the proposed method, experiments on a tissue-mimicking phantom are carried out. The results demonstrate that the proposed approach is capable of visualizing and quantifying the FRET distribution in scattering media, which implies the further application of the ratiometric assay in in vivo studies.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Fluorescence Resonance Energy Transfer / methods*
  • Green Fluorescent Proteins
  • HEK293 Cells
  • Humans
  • Imaging, Three-Dimensional / methods*
  • Optics and Photonics / methods
  • Phantoms, Imaging
  • Plasmids
  • Transfection

Substances

  • Green Fluorescent Proteins