Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy

Chem Commun (Camb). 2012 Sep 18;48(72):9059-61. doi: 10.1039/c2cc34379g. Epub 2012 Aug 2.

Abstract

C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Aptamers, Nucleotide / genetics
  • Base Sequence
  • Biosensing Techniques / methods*
  • Cyclic GMP / analogs & derivatives*
  • Cyclic GMP / analysis
  • RNA, Plant / chemistry
  • Riboswitch
  • Spectrometry, Fluorescence

Substances

  • Aptamers, Nucleotide
  • RNA, Plant
  • Riboswitch
  • bis(3',5')-cyclic diguanylic acid
  • Cyclic GMP