Abstract
C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Aptamers, Nucleotide / chemistry*
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Aptamers, Nucleotide / genetics
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Base Sequence
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Biosensing Techniques / methods*
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Cyclic GMP / analogs & derivatives*
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Cyclic GMP / analysis
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RNA, Plant / chemistry
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Riboswitch
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Spectrometry, Fluorescence
Substances
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Aptamers, Nucleotide
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RNA, Plant
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Riboswitch
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bis(3',5')-cyclic diguanylic acid
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Cyclic GMP