[Performance of the VIDAS automated immunoassay for the determination of Epstein-Barr virus serological status]

Fernando De Ory; María Eulalia Guisasola; Juan Carlos Sanz; Isabel García-Bermejo

doi:10.1016/j.eimc.2012.06.001

[Performance of the VIDAS automated immunoassay for the determination of Epstein-Barr virus serological status]

Enferm Infecc Microbiol Clin. 2012 Dec;30(10):618-20. doi: 10.1016/j.eimc.2012.06.001. Epub 2012 Jul 31.

[Article in Spanish]

Authors

Fernando De Ory¹, María Eulalia Guisasola, Juan Carlos Sanz, Isabel García-Bermejo

Affiliation

¹ Servicio de Microbiología Diagnóstica, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, España. fory@isciii.es

PMID: 22854635
DOI: 10.1016/j.eimc.2012.06.001

Abstract

Enzyme linked fluorescent assays (VIDAS EBV VCA IgM, VIDAS EBV VCA/EA IgG and VIDAS EBV EBNA IgG (Biomérieux, France) were evaluated to determine markers for infection of Epstein Barr virus, as well as to establish antibody profiles, compared with immunofluorescence assays as reference. The assays evaluated showed good values for sensitivity, specificity and agreement, making them useful for their application in clinical laboratories.

Publication types

Evaluation Study

MeSH terms

Antibodies, Viral / blood*
Antibodies, Viral / immunology
Antigens, Viral / immunology*
Automation
Capsid Proteins / immunology*
Enzyme-Linked Immunosorbent Assay / methods*
Epstein-Barr Virus Infections / diagnosis*
Epstein-Barr Virus Nuclear Antigens / immunology*
Fluorescent Antibody Technique, Indirect
Herpesvirus 4, Human / immunology*
Humans
Immunoglobulin G / blood*
Immunoglobulin G / immunology
Immunoglobulin M / blood*
Immunoglobulin M / immunology
Predictive Value of Tests
Sensitivity and Specificity

Substances

Antibodies, Viral
Antigens, Viral
Capsid Proteins
Epstein-Barr Virus Nuclear Antigens
Epstein-Barr viral capsid antigen
Immunoglobulin G
Immunoglobulin M