Abstract
Water-soluble poly(L-serine)s bearing long side-chain with terminal charge groups were synthesized via ring-opening polymerization of O-pentenyl-L-serine N-carboxyanhydride followed by thiol-ene reactions. These side-chain modified poly(L-serine)s adopt β-sheet conformation in aqueous solution with excellent stability against changes in pH and temperature. These water-soluble poly(L-serine) derivatives with charged side-chain functional groups and stable β-sheet conformations showed membrane-penetrating capabilities in different cell lines with low cytotoxicity.
Publication types
-
Research Support, N.I.H., Extramural
MeSH terms
-
Animals
-
Cell Line
-
Cell Membrane / drug effects
-
Cell Membrane / metabolism*
-
Cell Membrane Permeability / drug effects
-
Cell Survival / drug effects
-
Endocytosis / drug effects
-
Fluorescent Dyes
-
Genistein / pharmacology
-
HeLa Cells
-
Humans
-
Hydrogen-Ion Concentration
-
Macrophages / cytology
-
Macrophages / drug effects
-
Magnetic Resonance Spectroscopy
-
Mice
-
Peptides / chemical synthesis*
-
Peptides / metabolism*
-
Peptides / pharmacology
-
Protein Structure, Secondary
-
Rhodamines
-
Solubility
-
Static Electricity
-
Temperature
-
Water
-
beta-Cyclodextrins / pharmacology
Substances
-
Fluorescent Dyes
-
Peptides
-
Rhodamines
-
beta-Cyclodextrins
-
Water
-
polyserine
-
Genistein
-
betadex