A cysteine probe with high selectivity and sensitivity promoted by response-assisted electrostatic attraction

Chem Commun (Camb). 2012 Sep 11;48(70):8793-5. doi: 10.1039/c2cc33971d. Epub 2012 Jul 26.

Abstract

A new turn-on fluorescent probe for fast detection of cysteine in physiological conditions, based on a new response-assisted electrostatic attraction strategy, is reported. The practical utility of the probe in fluorescent protein labeling and subcellular imaging is also demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coumarins / chemistry*
  • Cysteine / chemistry*
  • Fluorescent Dyes / chemical synthesis*
  • Glutathione / chemistry
  • HeLa Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • Molecular Imaging
  • Proteins / analysis*
  • Proteins / chemistry
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence
  • Staining and Labeling
  • Static Electricity
  • Sulfhydryl Compounds / chemistry

Substances

  • Coumarins
  • Fluorescent Dyes
  • Proteins
  • Sulfhydryl Compounds
  • Glutathione
  • Cysteine