Photobiocatalyzed asymmetric reduction of ketones using Chlorella sp. MK201

Biotechnol Lett. 2012 Nov;34(11):2083-6. doi: 10.1007/s10529-012-1008-2. Epub 2012 Jul 25.

Abstract

Aromatic ketones were reduced using suspension culture of Chlorella sp. MK201 under fluorescent light illumination producing the corresponding chiral alcohols in high yields with excellent enantiomeric excess (ee). For example, 2',3',4',5',6'-pentafluoroacetophenone at 0.25 mg/ml was converted to the corresponding (S)-alcohol in 80 % yield with >99 % ee by 1 mg dry wt of Chlorella/ml in 12 h illumination (2,000 lux).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohols / chemistry
  • Alcohols / metabolism
  • Chlorella / metabolism*
  • Fluorescence
  • Ketones / chemistry*
  • Ketones / metabolism*
  • Oxidation-Reduction
  • Photochemical Processes
  • Stereoisomerism

Substances

  • Alcohols
  • Ketones