Brain isozyme of glycogen phosphorylase: immunohistological localization within the central nervous system

Brain Res. 1990 Oct 8;529(1-2):42-9. doi: 10.1016/0006-8993(90)90809-p.

Abstract

An antibody specific for the predicted carboxyterminal sequence of the human brain isozyme of glycogen phosphorylase (alpha-1,4-D-glucan:orthophosphate D-glucosyltransferase, EC 2.4.1.1) was generated to verify the carboxyterminal amino acid sequence of this protein. The isozyme-specific antibody was used to examine the localization of this protein in primate and non-primate brain. The highest levels of the brain isozyme in cerebrum and cerebellum were found in fibrous astrocytes, many with glial processes that appear to terminate upon blood vessels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Astrocytes / enzymology
  • Brain / enzymology*
  • Cerebellum / enzymology
  • Cerebral Cortex / enzymology
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoblotting
  • Immunoenzyme Techniques
  • Isoenzymes / analysis*
  • Isoenzymes / immunology
  • Macaca
  • Nerve Fibers, Myelinated
  • Phosphorylases / analysis*
  • Phosphorylases / immunology
  • Rats

Substances

  • Isoenzymes
  • Phosphorylases