Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus

Brain Res. 1990 Oct 8;529(1-2):302-8. doi: 10.1016/0006-8993(90)90841-x.

Abstract

A monoclonal antibody (mAb L6) to a carcinoma surface antigen has previously been shown to recognize neurophysins (NP), proteins associated with oxytocin and vasopressin. L6-reactivity in rat hypothalamus was confined to magnocellular neuronal systems. No staining was detected in parvicellular suprachiasmatic or paraventricular systems. mAb L6 immunoprecipitated vasopressin-neurophysin only under reducing conditions, and detected it in Western blots only after gel-renaturation and electroblotting in basic buffer. These findings suggest L6-reactivity to NP is conformation-sensitive, and imply NP expression in a unique configurational form in hypothalamic parvicellular systems.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Hypothalamus / chemistry
  • Hypothalamus / cytology*
  • Immunoblotting
  • Immunohistochemistry
  • Male
  • Median Eminence / cytology
  • Molecular Weight
  • Neurons / cytology*
  • Neurophysins / analysis*
  • Rats

Substances

  • Antibodies, Monoclonal
  • Neurophysins