Abstract
We report a novel continuous and sensitive fluorescence turn-on assay for ACPs, which consists of a cationic conjugated polyelectrolyte (PPE4+) and a commonly used phosphatase substrate p-nitrophenyl phosphate (pNPP). The kinetics of the ACP catalyzed hydrolysis of the substrate pNPP was monitored by the fluorescence change of PPE4+ and corresponding kinetic parameters were derived to be consistent with the literature reports. The applications of PPE4+/pNPP-based ACP assay in high-throughput screening of ACP inhibitors and detection of prostatic acid phosphotase (PAP) in vitro were demonstrated.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase / chemistry*
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Aniline Compounds / chemistry
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Catalysis
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Drug Design
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Electrolytes / chemistry*
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Humans
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Hydrolysis
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Kinetics
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Male
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Models, Chemical
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Nitrophenols / chemistry*
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Organophosphorus Compounds / chemistry*
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Phosphoric Monoester Hydrolases / chemistry
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Polymers / chemistry
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Prostatic Neoplasms / diagnosis
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Protein Tyrosine Phosphatases / chemistry
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Spectrometry, Fluorescence / methods
Substances
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Aniline Compounds
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Electrolytes
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Nitrophenols
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Organophosphorus Compounds
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Polymers
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nitrophenylphosphate
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4-aminophenylphosphate
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Acid Phosphatase
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Phosphoric Monoester Hydrolases
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prostatic acid phosphatase
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Protein Tyrosine Phosphatases