Live-cell super-resolution imaging goes multicolor

Chembiochem. 2012 Sep 3;13(13):1861-3. doi: 10.1002/cbic.201200347. Epub 2012 Jul 13.

Abstract

New resolutions: The combined use of photoactivatable fluorescent proteins and synthetic fluorophores considerably expands our options for multicolor super-resolution fluorescence imaging and enables for the first time the simultaneous imaging of more than two proteins with subdiffraction optical resolution in living cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Survival
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / metabolism
  • HeLa Cells
  • Humans
  • Luminescent Proteins / analysis
  • Luminescent Proteins / metabolism
  • Microscopy, Fluorescence / methods*
  • Models, Molecular
  • Proteins / analysis*
  • Proteins / metabolism

Substances

  • Fluorescent Dyes
  • Luminescent Proteins
  • Proteins