Activity-based near-infrared fluorescent probe for LMP7: a chemical proteomics tool for the immunoproteasome in living cells

Chembiochem. 2012 Sep 3;13(13):1899-903. doi: 10.1002/cbic.201200307. Epub 2012 Jul 17.

Abstract

Probing the unknown: The immunoproteasome, an alternative form of the constitutive proteasome, has been implicated in a number of pathological states such as cancer and autoimmune diseases. In an effort to understand the role of the immunoproteasome in cells, the first immunoproteasome-specific near-infrared fluorescent probe has been developed.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Binding Sites
  • Cell Line
  • Cell Line, Tumor
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry
  • Humans
  • Microscopy, Fluorescence
  • Molecular Docking Simulation
  • Proteasome Endopeptidase Complex / analysis*
  • Proteasome Endopeptidase Complex / immunology
  • Proteomics / methods

Substances

  • Fluorescent Dyes
  • LMP7 protein
  • Proteasome Endopeptidase Complex