Natural fruit extracts as non-toxic fluorescent dyes for staining fungal chlamydospores

World J Microbiol Biotechnol. 2012 Jan;28(1):387-90. doi: 10.1007/s11274-011-0815-2. Epub 2011 Jun 21.

Abstract

Currently, most synthetic dyes utilized for fungal fluorescent staining are toxic, carcinogenic, or harmful to animals, humans, and the environment. This study proposes non-toxic extracts of fruits from the genera Rhamnus, Ribes, Sambucus, Viburnum, Sorbus and Beta as simple, safe, and ecological alternatives to chemical fluorescent dye for efficient staining of Fusarium chlamydospore cells using, as test strains, five different pathogenic Fusarium species.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes / isolation & purification*
  • Food Contamination / prevention & control
  • Food Microbiology
  • Food Technology
  • Fruit / chemistry*
  • Fusarium / isolation & purification
  • Fusarium / pathogenicity*
  • Microscopy, Fluorescence
  • Plant Extracts / chemistry
  • Spores, Fungal / isolation & purification
  • Staining and Labeling / methods*

Substances

  • Fluorescent Dyes
  • Plant Extracts