[Discussion of indoor quality control method of MPAIA antibody test of Schistosoma japonicum]

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2012 Apr;24(2):150-4.
[Article in Chinese]

Abstract

Objective: To explore the scientific and effective indoor quality control method to ensure the results of Magnetic Particle Antibody Immunoassay (MPAIA) antibody test of Schistosoma japonicum reliable.

Methods: The MPAIA antibody test indoor quality control work was performed with the combination of the crubbs method, Z-scores figure and Westgard multi-rules quality control method.

Results: The crubbs test entered the quality control condition state from the third times, after 20 times, the test switched to Z-scores conventional quality control, and the Westgard multi-rules quality control method could help analyze the type of errors. From the third to fortieth test, 186 samples were completed and the tests of 9 samples among 2 batches needed to be re-done because of the failure of quality control. The test results of other 177 samples were acceptable.

Conclusion: The combination of the crubbs method, Z-scores figure and Westgard multi-rules quality control method can effectively accomplish the indoor quality control of MPAIA antibody test of Schistosoma japonicum.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Antibodies, Helminth / immunology*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / standards
  • Humans
  • Quality Control
  • Schistosoma japonicum / immunology*
  • Schistosomiasis japonica / diagnosis*
  • Schistosomiasis japonica / immunology*

Substances

  • Antibodies, Helminth