Backbone modification promotes peroxidase activity of G-quadruplex-based DNAzyme

Chem Commun (Camb). 2012 Aug 28;48(67):8347-9. doi: 10.1039/c2cc32919k. Epub 2012 Jul 13.

Abstract

In this work we studied how backbone chemical modifications, such as 2'-O-methyl, phosphorothioate, L-form nucleotides and locked nucleic acid, on G-quadruplex based DNAzymes would affect their peroxidase activity. Our results indicate that 2'-O-methyl modification facilitates the formation of a perfectly compacted parallel structure and significantly promotes peroxidase activity of G-quadruplex based DNAzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • DNA, Catalytic / chemistry*
  • DNA, Catalytic / metabolism*
  • Enzyme Activation
  • Enzyme Stability
  • G-Quadruplexes*
  • Models, Molecular
  • Molecular Structure
  • Peroxidases / metabolism*

Substances

  • DNA, Catalytic
  • Peroxidases