Abstract
A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Assay*
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Cell Nucleus / drug effects*
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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Dose-Response Relationship, Drug
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Gene Expression / drug effects
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Genes, Reporter
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HeLa Cells
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Humans
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Luciferases, Renilla
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Pyrans / pharmacology*
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RNA Precursors / antagonists & inhibitors
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RNA Precursors / genetics
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RNA Splicing / drug effects*
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RNA Splicing / genetics
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RNA, Messenger / antagonists & inhibitors*
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RNA, Messenger / genetics
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Spiro Compounds / pharmacology*
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Spliceosomes / drug effects
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Spliceosomes / genetics
Substances
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Pyrans
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RNA Precursors
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RNA, Messenger
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Spiro Compounds
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spliceostatin A
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Luciferases, Renilla