Molecular analysis of the human mitochondrial DNA control region for forensic identity testing

Curr Protoc Hum Genet. 2012 Jul:Chapter 14:Unit14.7. doi: 10.1002/0471142905.hg1407s74.

Abstract

This unit highlights methods used to perform PCR amplification and sequence analysis of mitochondrial DNA (mtDNA) on pristine and highly degraded biological material. The focus is on applications to forensic casework, and a number of case examples are provided. Any laboratory working with DNA from old or "ancient" samples will benefit from this information.

MeSH terms

  • DNA Fingerprinting / methods*
  • DNA, Mitochondrial / analysis*
  • Forensic Anthropology / methods
  • Forensic Genetics / methods*
  • Humans
  • Mitochondria / genetics
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA / methods

Substances

  • DNA, Mitochondrial