Detection of carbapenemases in Enterobacteriaceae by a commercial multiplex PCR

J Clin Microbiol. 2012 Sep;50(9):3115-8. doi: 10.1128/JCM.00991-12. Epub 2012 Jul 11.

Abstract

A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacteriological Techniques / methods*
  • Enterobacteriaceae / enzymology*
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • Reagent Kits, Diagnostic
  • beta-Lactamases
  • carbapenemase