Expression of differentiation inhibiting factor 1 and vascular endothelial growth factor and the relation between them and microvessel density in gastric cancer tissue

Hepatogastroenterology. 2013 Jan-Feb;60(121):197-9. doi: 10.5754/hge12511.

Abstract

Background/aims: To observe the expression of differentiation inhibiting factor 1 (DIF1) and vascular endothelial growth factor (VEGF) and explore the relation between them and microvessel density (MVD) in gastric cancer tissue.

Methodology: The expression of DIF1, VEGF, CD34 and MVD in gastric cancer and peri-cancerous tissues was qualitatively analyzed with immunohistochemistry. The protein expression of DIF1 and VEGF in gastric cancer and peri-cancerous tissue was semi-quantitatively analyzed with Western blot.

Results: The expression of DIF1 and VEGF, and MVD were significantly higher in gastric cancer tissue than in peri-cancerous tissue (p<0.05). MVD was significantly higher in DIF-positive gastric cancer tissue than in DIF-negative gastric cancer tissue (p<0.001).

Conclusions: In gastric cancer, DIF1 expression is related to VEGF expression and MVD. DIF1 may promote angiogenesis in gastric cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / analysis
  • Blotting, Western
  • Female
  • Humans
  • Male
  • Microvessels / pathology*
  • Stomach Neoplasms / blood supply*
  • Stomach Neoplasms / drug therapy
  • Stomach Neoplasms / pathology
  • Vascular Endothelial Growth Factor A / analysis*
  • Vascular Endothelial Growth Factor A / physiology

Substances

  • Antigens, CD34
  • Vascular Endothelial Growth Factor A