Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

Arash Ghodousi; S Vatani; Davood Darban-Sarokhalil; Maryam Omrani; A Fooladi; A Khosaravi; Mohammad Mehdi Feizabadi

Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

Iran J Microbiol. 2012 Mar;4(1):44-6.

Authors

Arash Ghodousi¹, S Vatani, Davood Darban-Sarokhalil, Maryam Omrani, A Fooladi, A Khosaravi, Mohammad Mehdi Feizabadi

Affiliation

¹ Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 22783461
PMCID: PMC3391561

Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

Keywords: Mycobacterium tuberculosis; Pulsed-filed gel electrophoresis; TB-complex.