Study of interaction of long-chain n-alcohols with fluid DOPC bilayers by a lateral pressure sensitive fluorescence probe

Gen Physiol Biophys. 2012 Jun;31(2):225-7. doi: 10.4149/gpb_2012_021.

Abstract

The excimer 1,2-dipyrenedecanoyl-sn-glycero-3-phosphatidylcholine (dipy10PC) fluorescence probe was used to determine effects of aliphatic alcohols (CnH2n+1OH, n = 12-18 is the even number of carbons in alkyl chain) on fluid dioleoylphosphatidylcholine (DOPC) +dioleoylphosphatidylserine (DOPS) bilayers in multilamellar vesicles at molar ratio DOPC/DOPS = 24.7. The excimer to monomer fluorescence intensity ratio increases with the increase of CnH2n+1OH/DOPC molar ratio and decreases with the CnH2n+1OH alkyl chain length n at a constant CnH2n+1OH/DOPC = 0.4 molar ratio. These effects indicate changes in the bilayer lateral pressure on the level of pyrenyl moieties location.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohols / chemistry*
  • Fluorescent Dyes / chemistry*
  • Lipid Bilayers / chemistry*
  • Phosphatidylcholines / chemistry*
  • Pressure
  • Spectrometry, Fluorescence / methods*

Substances

  • Alcohols
  • Fluorescent Dyes
  • Lipid Bilayers
  • Phosphatidylcholines
  • 1,2-oleoylphosphatidylcholine