Measurement of haloperidol reductase activity in red blood cells and reduced haloperidol/haloperidol ratios in plasma in oriental psychiatric patients

M Shibasaki; T Someya; T Kato; T Noguchi; N Ishida; S Takahashi

doi:10.1016/0278-5846(90)90079-v

Measurement of haloperidol reductase activity in red blood cells and reduced haloperidol/haloperidol ratios in plasma in oriental psychiatric patients

Prog Neuropsychopharmacol Biol Psychiatry. 1990;14(6):941-7. doi: 10.1016/0278-5846(90)90079-v.

Authors

M Shibasaki¹, T Someya, T Kato, T Noguchi, N Ishida, S Takahashi

Affiliation

¹ Department of Psychiatry, Shiga University of Medical Science, Otsu, Japan.

PMID: 2277853
DOI: 10.1016/0278-5846(90)90079-v

Abstract

1. The authors established a method for measuring haloperidol (HAL) reductase activity in human red blood cells. 2. Characteristics of the HAL reductase in red blood cells were examined. This enzyme reaction was NADPH dependent, and the optimum pH was at 8.2-8.9. Vmax and Km were calculated as 25-150 pmol/hr/10(6) RBC and 160-2600 microM respectively. 3. HAL reductase activities in red blood cells from 14 patients treated with HAL were in a range of 9.7-20.8 pmol/hr/10(6) RBC. So far we did not find any significant correlation between HAL reductase activities and reduced HAL/HAL ratios in plasma.

MeSH terms

Adult
Alcohol Oxidoreductases / blood*
Delusions / blood*
Delusions / drug therapy
Erythrocytes / enzymology*
Female
Haloperidol / blood*
Haloperidol / therapeutic use
Humans
Kinetics
Male
Reference Values
Schizophrenia / blood*
Schizophrenia / drug therapy

Substances

Alcohol Oxidoreductases
haloperidol reductase
Haloperidol