Abstract
Color bind: We have developed a probe TMR-para-MG that switches its fluorescence emission upon binding to a fluorogen-activating protein (FAP). In cells that express FAP, this dye labels target sites in one color and mitochondria in another color, thus it might be a suitable tool for monitoring changes in mitochondrial membrane potential.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Color*
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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HeLa Cells
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Humans
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Membrane Potential, Mitochondrial
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mitochondria / metabolism
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Models, Molecular
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Molecular Structure
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Rhodamines / chemistry*
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Rhodamines / metabolism
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Rosaniline Dyes / chemical synthesis
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Rosaniline Dyes / chemistry*
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Rosaniline Dyes / metabolism
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Spectrometry, Fluorescence
Substances
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Fluorescent Dyes
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Membrane Proteins
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Rhodamines
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Rosaniline Dyes
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malachite green
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tetramethylrhodamine