Removal of nitrate using Paracoccus sp. YF1 immobilized on bamboo carbon

Abstract

Paracoccus sp. strain YF1 immobilized on bamboo carbon was developed for the denitrification. The results show that denitrification was significantly improved using immobilized cells compared to that of free cells, where denitrification time decreased from 24h (free cells) to 15 h (immobilized cells). The efficiency of denitrification increased from 4.57 mg/(Lh) (free cells) to 6.82 mg/(Lh) (immobilized cells). Kinetics studies suggest that denitrification by immobilized YF1 cells fitted well to the zero-order model. Scanning electron microscopy (SEM) demonstrated that firstly, the bacteria became stable on the inside and exterior of the bamboo carbon particles and secondly, they formed biofilm after adhesion. Various factors and their influences on biological denitrification were investigated, namely temperature, pH, initial nitrate concentrations and carbon sources. The immobilized cells exhibited more nitrate removal at various conditions compared to free cells since bamboo carbon as a carrier protects cells against changes in environmental conditions. Denitrification using the YF1 immobilized in bamboo carbon was also maintained 99.8% after the tenth cycle reuse, thus demonstrating excellent reusability. Finally, wastewater was treated using the immobilized cells and the outcome was that nitrogen was completely removed by bamboo-immobilized YF1.