Abstract
This study tested the hypothesis that CD4(+)CD25(+)CD127(low) regulatory T (Treg) cells might induce immunosuppressive properties in apoptotic neutrophils. Treg cells are recognized as a major subset of immune cells possessing potent suppressive properties directed at T effector cells. However, Treg cells have recently been found to inhibit neutrophil function and promote their apoptosis. One of the mechanisms of action of Treg cells is the induction of other suppressor cell populations according to an infectious tolerance model. We showed that LPS-activated Treg cells promote generation of IL-10 and TGF-β1, inhibit IL-6 production by PMNs and induce the expression of heme oxygenase-1 (HO-1) and the suppressor of cytokine signaling 3 molecule (SOCS3). However, CD3/CD28-activated Treg cells were seen to promote TGF-β1 production, as well as IDO and HO-1 expression by PMNs. These findings suggest that Treg cells might play an important role in the direct control of innate immune responses through the induction of neutrophils with immunosuppressive properties that generate IL-10, TGF-β1, IDO and HO-1.
Copyright © 2012 Elsevier GmbH. All rights reserved.
Publication types
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Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects
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Apoptosis / immunology
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Cell Communication / drug effects
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Cell Communication / immunology*
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Female
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Heme Oxygenase-1 / immunology
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Humans
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Immune Tolerance / drug effects
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Immune Tolerance / physiology*
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Immunity, Innate / drug effects
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Immunity, Innate / physiology*
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Indoleamine-Pyrrole 2,3,-Dioxygenase / immunology
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Interleukin-10 / immunology
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Interleukin-6 / immunology
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Lipopolysaccharides / pharmacology
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Lymphocyte Activation / drug effects
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Lymphocyte Activation / immunology
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Male
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Neutrophils / cytology
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Neutrophils / immunology*
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Suppressor of Cytokine Signaling 3 Protein
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Suppressor of Cytokine Signaling Proteins / immunology
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T-Lymphocytes, Regulatory / cytology
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T-Lymphocytes, Regulatory / immunology*
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Transforming Growth Factor beta1 / immunology
Substances
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IL10 protein, human
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IL6 protein, human
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Indoleamine-Pyrrole 2,3,-Dioxygenase
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Interleukin-6
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Lipopolysaccharides
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SOCS3 protein, human
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Suppressor of Cytokine Signaling 3 Protein
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Suppressor of Cytokine Signaling Proteins
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TGFB1 protein, human
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Transforming Growth Factor beta1
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Interleukin-10
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HMOX1 protein, human
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Heme Oxygenase-1