Construction of plasmid-free derivatives of Salmonella typhimurium LT2 using temperature-sensitive mutants of pKZ1 for displacement of the resident plasmid, pSLT

R A Kelln; L G Lintott

doi:10.1007/BF00633852

Construction of plasmid-free derivatives of Salmonella typhimurium LT2 using temperature-sensitive mutants of pKZ1 for displacement of the resident plasmid, pSLT

Mol Gen Genet. 1990 Jul;222(2-3):438-40. doi: 10.1007/BF00633852.

Authors

R A Kelln¹, L G Lintott

Affiliation

¹ Department of Chemistry, University of Regina, Saskatchewan, Canada.

PMID: 2274042
DOI: 10.1007/BF00633852

Abstract

Replication (or partitioning) temperature-sensitive mutants of pKZ1 were isolated and shown to exhibit incompatibility with the resident plasmid (pSLT) of Salmonella typhimurium LT2. Following displacement of pSLT, the mutant plasmids were effectively eliminated from the cell population by passage at 42 degrees C, yielding plasmid-free isolates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Southern
Conjugation, Genetic
DNA, Bacterial / biosynthesis
Kanamycin Resistance / genetics
Mutation
Plasmids*
Salmonella typhimurium / drug effects
Salmonella typhimurium / genetics*
Temperature
Transformation, Bacterial

Substances

DNA, Bacterial