Abstract
Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbocyanines
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Color
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Escherichia coli / genetics
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes
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Humans
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Hydrazines
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Molecular Imaging / methods*
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Nuclear Pore Complex Proteins / chemistry*
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Nuclear Pore Complex Proteins / genetics
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Organic Chemicals
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Protein Conformation
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae Proteins / chemistry*
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Saccharomyces cerevisiae Proteins / genetics
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Spectrometry, Fluorescence
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Staining and Labeling / methods*
Substances
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Alexa 488 hydrazide
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Alexa Fluor 647
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Alexa594
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Carbocyanines
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Fluorescent Dyes
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Hydrazines
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NUP49 protein, S cerevisiae
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Nuclear Pore Complex Proteins
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Organic Chemicals
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins