Development of reversible fluorescence probes based on redox oxoammonium cation for hypobromous acid detection in living cells

Chem Commun (Camb). 2012 Aug 11;48(62):7735-7. doi: 10.1039/c2cc33264g. Epub 2012 Jun 27.

Abstract

We describe the synthesis, properties, and application of two reversible fluorescent probes, mCy-TemOH and Cy-TemOH, for HOBr sensing and imaging in live cells. The two probes contain a hydroxylamine functional group for the monitoring of HOBr oxidation/ascorbic acid reduction events. Confocal fluorescence microscopy has established the HOBr detection in live-cells.

MeSH terms

  • Animals
  • Ascorbic Acid / chemistry
  • Bromates / analysis*
  • Bromides / pharmacology
  • Cell Line
  • Cyclic N-Oxides / chemical synthesis*
  • Eosinophil Peroxidase / metabolism
  • Eosinophil Peroxidase / pharmacology
  • Fluorescent Dyes / chemical synthesis*
  • Hydrogen Peroxide / pharmacology
  • Hydroxylamines / chemistry
  • Indoles / chemical synthesis*
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Mice
  • Microscopy, Confocal
  • Molecular Imaging / methods*
  • Oxidation-Reduction
  • Potassium Compounds / pharmacology
  • Spectrometry, Fluorescence

Substances

  • Bromates
  • Bromides
  • Cyclic N-Oxides
  • Fluorescent Dyes
  • Hydroxylamines
  • Indoles
  • Potassium Compounds
  • Hydrogen Peroxide
  • Eosinophil Peroxidase
  • hypobromous acid
  • potassium bromide
  • Ascorbic Acid