Abstract
We describe the synthesis, properties, and application of two reversible fluorescent probes, mCy-TemOH and Cy-TemOH, for HOBr sensing and imaging in live cells. The two probes contain a hydroxylamine functional group for the monitoring of HOBr oxidation/ascorbic acid reduction events. Confocal fluorescence microscopy has established the HOBr detection in live-cells.
MeSH terms
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Animals
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Ascorbic Acid / chemistry
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Bromates / analysis*
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Bromides / pharmacology
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Cell Line
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Cyclic N-Oxides / chemical synthesis*
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Eosinophil Peroxidase / metabolism
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Eosinophil Peroxidase / pharmacology
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Fluorescent Dyes / chemical synthesis*
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Hydrogen Peroxide / pharmacology
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Hydroxylamines / chemistry
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Indoles / chemical synthesis*
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Macrophages / cytology
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Macrophages / drug effects
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Macrophages / metabolism*
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Mice
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Microscopy, Confocal
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Molecular Imaging / methods*
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Oxidation-Reduction
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Potassium Compounds / pharmacology
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Spectrometry, Fluorescence
Substances
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Bromates
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Bromides
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Cyclic N-Oxides
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Fluorescent Dyes
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Hydroxylamines
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Indoles
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Potassium Compounds
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Hydrogen Peroxide
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Eosinophil Peroxidase
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hypobromous acid
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potassium bromide
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Ascorbic Acid