Objective: To increase the recombinant adenovirus vector mediated human rotavirus VP6 gene expression through coden optimization.
Methods: We have artificially synthesized VP6 gene of group A human rotavirus according to the human biased codon. The modified gene was transfected into 293 cells using adenovirus vector and the gene product, the respective protein was produced. The expression level of optimized gene and wild type gene was detected by Immunofluorescence (IF) and Western Blot.
Result: A remarkable increase of the expression level of optimized VP6 gene in comparison with the wild-type control.
Conclusion: The coden optimization indeed help increasing the recombinant adenovirus mediated human rotavirus gene expression, which indicated the potential application of such recombinant adenoviruses in the development of adenoviral-vectored rotavirus vaccines.