Abstract
The search for a specific rRNA methylase motif led to the identification of the new macrolide, lincosamide, and streptogramin B resistance gene erm(43) in Staphylococcus lentus. An inducible resistance phenotype was demonstrated by cloning and expressing erm(43) and its regulatory region in Staphylococcus aureus. The erm(43) gene was detected in two different DNA fragments, of 6,230 bp and 1,559 bp, that were each integrated at the same location in the chromosome in several S. lentus isolates of human, dog, and chicken origin.
MeSH terms
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Adhesins, Bacterial / chemistry
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Adhesins, Bacterial / classification
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Adhesins, Bacterial / genetics*
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Adhesins, Bacterial / metabolism
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology
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Chickens
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Chromosomes, Bacterial*
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Cloning, Molecular
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DNA, Bacterial*
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Dogs
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Drug Resistance, Bacterial
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Humans
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Lincosamides / chemistry
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Lincosamides / pharmacology
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Macrolides / chemistry
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Macrolides / pharmacology
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Methyltransferases / chemistry
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Methyltransferases / classification
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Methyltransferases / genetics*
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Methyltransferases / metabolism
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Molecular Sequence Data
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Phylogeny
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Alignment
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Staphylococcal Infections / drug therapy
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Staphylococcal Infections / microbiology
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Staphylococcus / classification
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Staphylococcus / enzymology
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Staphylococcus / genetics*
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Staphylococcus aureus / enzymology
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Staphylococcus aureus / genetics
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Streptogramin B / chemistry
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Streptogramin B / pharmacology
Substances
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Adhesins, Bacterial
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Anti-Bacterial Agents
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DNA, Bacterial
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Lincosamides
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Macrolides
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Recombinant Proteins
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Streptogramin B
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Methyltransferases
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rRNA (adenosine-O-2'-)methyltransferase