A universal platform for amplified multiplexed DNA detection based on exonuclease III-coded magnetic microparticle probes

Chem Commun (Camb). 2012 Jul 28;48(59):7416-8. doi: 10.1039/c2cc33249c. Epub 2012 Jun 20.

Abstract

An amplified multiplexed DNA detection biosensor has been developed, which combines the unique cleavage function of exonuclease III (Exo III) with the separating ability of magnetic microparticles (MMPs). By using different fluorophores, the multiplexed detection of DNA is demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biosensing Techniques*
  • DNA, Viral / analysis*
  • Ebolavirus / genetics*
  • Exodeoxyribonucleases / chemistry
  • Fluorescent Dyes / chemistry
  • HIV / genetics*

Substances

  • DNA, Viral
  • Fluorescent Dyes
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III