We present novel experimental method for estimation of the light penetration depth (LPD) in turbid media based on the analysis of the speckle pattern structure. Under the certain illumination conditions this structure is strongly dependent on the penetration depth. Presented theoretical model based on the Bragg diffraction from the thick holograms allows LPD estimation if only one parameter of the material, namely refractive index, of the material is known. Feasibility of the method was checked experimentally. Experimental results obtained for variety of the materials are in good agreement with the theoretical assumptions. It was shown that qualitative LPD comparison does not require knowledge of the material properties.