Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex

Seng Fook Koh; Sun Tee Tay; Rasana Sermswan; Surasakdi Wongratanacheewin; Kek Heng Chua; Savithri D Puthucheary

doi:10.1016/j.mimet.2012.06.002

Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex

J Microbiol Methods. 2012 Sep;90(3):305-8. doi: 10.1016/j.mimet.2012.06.002. Epub 2012 Jun 15.

Authors

Seng Fook Koh¹, Sun Tee Tay, Rasana Sermswan, Surasakdi Wongratanacheewin, Kek Heng Chua, Savithri D Puthucheary

Affiliation

¹ Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia. kohsf.adrian@gmail.com

PMID: 22705921
DOI: 10.1016/j.mimet.2012.06.002

Abstract

We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Burkholderia cepacia complex / genetics*
Burkholderia mallei / genetics*
Burkholderia pseudomallei / genetics*
Electrophoresis, Agar Gel
Genes, Bacterial
Molecular Typing
Multiplex Polymerase Chain Reaction*
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics

Substances

RNA, Bacterial
RNA, Ribosomal, 16S