Abstract
A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our results suggest that VP2 protein expressed from MVA-VP2 maintained the correct conformational structure. To our knowledge, this is the first report on the usefulness of MVA-based vectors for developing recombinant vaccines for poultry.
Publication types
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Controlled Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral
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Birnaviridae Infections / prevention & control
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Birnaviridae Infections / veterinary*
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Cells, Cultured
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Chick Embryo
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Chickens
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Fibroblasts / metabolism
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Infectious bursal disease virus / immunology*
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Poultry Diseases / prevention & control*
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Poultry Diseases / virology
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Specific Pathogen-Free Organisms
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Vaccinia virus / genetics*
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Vaccinia virus / immunology
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Vaccinia virus / metabolism
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Viral Structural Proteins / genetics
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Viral Structural Proteins / immunology*
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Viral Structural Proteins / metabolism
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Viral Vaccines / immunology*
Substances
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Antibodies, Viral
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VP2 protein, infectious bursal disease virus
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Viral Structural Proteins
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Viral Vaccines