Abstract
The aim of the present study was to determine the effect of baicalein on metabolic syndrome induced by a high-fat diet in mice. The mice developed obesity, dyslipidemia, fatty liver, diabetes and insulin resistance. These disorders were effectively normalized in baicalein-treated mice. Further investigation revealed that the inhibitory effect on inflammation and insulin resistance was mediated by inhibition of the MAPKs pathway and activation of the IRS1/PI3K/Akt pathway. The lipid-lowering effect was attributed to the blocking of synthesis way mediated by SERBP-1c, PPARγ and the increased fatty acid oxidation. All of these effects depended on AMPKα activation. These results were confirmed in the primary hepatocytes from wild type and AMPKα(2)(-/-) mice. However, the IRS-1/PI3K/AKT pathway showed no change, which may be due to the time of stimulation and concentration. Thus, these data suggested that baicalein protects mice from metabolic syndrome through an AMPKα(2)-dependent mechanism involving multiple intracellular signaling pathways.
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AMP-Activated Protein Kinases / metabolism*
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Animals
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Biosynthetic Pathways / genetics
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Blood Glucose
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Body Weight / drug effects
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Cells, Cultured
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Chemokine CCL2 / blood
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Diet, High-Fat / adverse effects*
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Drugs, Chinese Herbal / pharmacology*
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Drugs, Chinese Herbal / therapeutic use
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Energy Metabolism
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Enzyme Activators / pharmacology*
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Enzyme Activators / therapeutic use
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Fatty Acids / biosynthesis
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Fatty Liver / drug therapy
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Fatty Liver / etiology
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Fatty Liver / pathology
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Flavanones / pharmacology*
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Flavanones / therapeutic use
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Gene Expression / drug effects
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Hepatocytes / drug effects
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Hepatocytes / metabolism
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Insulin Resistance
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MAP Kinase Signaling System
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Male
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Metabolic Syndrome / drug therapy*
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Metabolic Syndrome / enzymology
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Metabolic Syndrome / etiology
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mitogen-Activated Protein Kinases / metabolism
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Obesity, Abdominal / drug therapy
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Obesity, Abdominal / enzymology
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Obesity, Abdominal / etiology
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Oxidative Stress
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Tumor Necrosis Factor-alpha / blood
Substances
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Blood Glucose
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Ccl2 protein, mouse
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Chemokine CCL2
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Drugs, Chinese Herbal
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Enzyme Activators
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Fatty Acids
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Flavanones
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Tumor Necrosis Factor-alpha
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baicalein
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AMPK alpha2 subunit, mouse
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Mitogen-Activated Protein Kinases
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AMP-Activated Protein Kinases