Four new cycloartane glycosides, 3-O-β-D-xylopyranosyl-(1 → 6)-β-D-glucopyranosyl-(1 → 4)-β-D-fucopyranosyl (22S,24Z)-cycloart-24-en-3β,22,26-triol 26-O-(6-O-acetyl)-β-D-glucopyranoside (1), 3-O-α-L-arabinopyranosyl-(1 → 6)-β-D-glucopyranosyl-(1 → 4)-β-D-fucopyranosyl (22S,24Z)-cycloart-24-en-3β,22,26-triol 26-O-(6-O-acetyl)-β-D-glucopyranoside (2), 3-O-β-D-glucopyranosyl (24S)-cycloartane-3β,16β,24,25,30-pentaol 25-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside (3) and 3-O-β-D-glucopyranosyl (24S)-cycloartane-3β,16β,24,25,30-pentaol 25-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside (4), were isolated from the aerial parts of Thalictrum fortunei. Their structures were established on the basis of extensive NMR and HR-ESI-MS analyses, along with acid hydrolysis.