Abstract
The ideal therapy for HIV infection requires a method to eliminate all HIV-harboring cells in the infected individual. The authors are developing an HIV-specific promoter to drive the expression of suicide genes that would induce cell death specifically in HIV-infected cells. The authors constructed a promoter that is 100-fold more responsive to the HIV transcriptional activator, Tat, than cellular transcription factors, using a plasmid expressing luciferase under the control of the mutated LTR promoter.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Death / genetics
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Gene Expression Regulation, Viral / genetics
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Genes, Transgenic, Suicide / genetics
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Genes, tat / genetics
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Genetic Therapy / methods*
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HIV Infections / therapy*
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HIV Long Terminal Repeat / genetics
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HIV-1 / genetics*
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HeLa Cells
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Humans
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Luciferases
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Luminescent Agents
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Mutation / genetics
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Plasmids / genetics
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Promoter Regions, Genetic / genetics
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T-Lymphocytes / virology*
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Transfection
Substances
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Luminescent Agents
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Luciferases