Two SusD-like proteins encoded within a polysaccharide utilization locus of an uncultured ruminant Bacteroidetes phylotype bind strongly to cellulose

A K Mackenzie; P B Pope; H L Pedersen; R Gupta; M Morrison; W G T Willats; V G H Eijsink

doi:10.1128/AEM.01164-12

Two SusD-like proteins encoded within a polysaccharide utilization locus of an uncultured ruminant Bacteroidetes phylotype bind strongly to cellulose

Appl Environ Microbiol. 2012 Aug;78(16):5935-7. doi: 10.1128/AEM.01164-12. Epub 2012 Jun 8.

Authors

A K Mackenzie¹, P B Pope, H L Pedersen, R Gupta, M Morrison, W G T Willats, V G H Eijsink

Affiliation

¹ Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.

Abstract

We demonstrate that two characteristic Sus-like proteins encoded within a polysaccharide utilization locus (PUL) bind strongly to cellulosic substrates and interact with plant primary cell walls. This shows associations between uncultured Bacteroidetes-affiliated lineages and cellulose in the rumen and thus presents new PUL-derived targets to pursue regarding plant biomass degradation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arabidopsis / microbiology
Bacterial Proteins / genetics
Bacterial Proteins / isolation & purification
Bacterial Proteins / metabolism*
Bacteroidetes / genetics*
Cell Wall / microbiology
Cellulose / metabolism*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Molecular Sequence Data
Protein Binding
Rumen / microbiology
Sequence Analysis, DNA

Substances

Bacterial Proteins
DNA, Bacterial
Cellulose

Associated data

GENBANK/JQ755420
GENBANK/JQ755421