Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides

FEBS Lett. 2012 Jul 30;586(16):2404-10. doi: 10.1016/j.febslet.2012.05.055. Epub 2012 Jun 7.

Abstract

Clostridium botulinum produces the botulinum neurotoxin, forming a large complex as progenitor toxins in association with non-toxic non-hemagglutinin and/or several different hemagglutinin (HA) subcomponents, HA33, HA17 and HA70, which bind to carbohydrate of glycoproteins from epithelial cells in the infection process. To elucidate the carbohydrate recognition mechanism of HA70, X-ray structures of HA70 from type C toxin (HA70/C) in complexes with sialylated oligosaccharides were determined, and a binding assay by the glycoconjugate microarray was performed. These results suggested that HA70/C can recognize both α2-3- and α2-6-sialylated oligosaccharides, and that it has a higher affinity for α2-3-sialylated oligosaccharides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Botulinum Toxins / chemistry*
  • Carbohydrate Sequence
  • Carbohydrates / chemistry*
  • Clostridium botulinum / metabolism*
  • Crystallography, X-Ray / methods
  • Dose-Response Relationship, Drug
  • Escherichia coli / metabolism
  • Galactose / chemistry
  • Glucose / chemistry
  • Glycoproteins / chemistry
  • Hemagglutinins / chemistry
  • Models, Molecular
  • Molecular Conformation
  • Molecular Sequence Data
  • N-Acetylneuraminic Acid / chemistry*
  • Oligosaccharides / chemistry*
  • Protein Binding
  • Temperature

Substances

  • Carbohydrates
  • Glycoproteins
  • Hemagglutinins
  • Oligosaccharides
  • Botulinum Toxins
  • botulinum toxin type C
  • N-Acetylneuraminic Acid
  • Glucose
  • Galactose

Associated data

  • PDB/4EN6
  • PDB/4EN7
  • PDB/4EN8
  • PDB/4EN9