Self-assembled magnetic bead biosensor for measuring bacterial growth and antimicrobial susceptibility testing

Small. 2012 Aug 20;8(16):2477-82. doi: 10.1002/smll.201200110. Epub 2012 Jun 5.

Abstract

Bacterial antibiotic resistance is one of the major concerns of modern healthcare worldwide, and the development of rapid, growth-based, antimicrobial susceptibility tests is key for addressing it. The cover image shows a self-assembled asynchronous magnetic bead rotation (AMBR) biosensor developed for rapid detection of bacterial growth. Using the biosensors, the minimum inhibitory concentration of a clinical E. coli isolate can be measured within two hours, where currently tests take 6-24 hours. A 16-well prototype is also constructed for simple and robust observation of the self-assembled AMBR biosensors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anti-Infective Agents / pharmacology*
  • Biosensing Techniques / instrumentation*
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development*
  • Escherichia coli / isolation & purification
  • Magnetics / instrumentation*
  • Microbial Sensitivity Tests / instrumentation*
  • Microbial Sensitivity Tests / methods*
  • Microspheres*
  • Rotation

Substances

  • Anti-Infective Agents