Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children

Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Mar;113(3):378-83. doi: 10.1016/j.oooo.2011.09.006.

Abstract

Objective: The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children.

Study design: To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120.

Results: Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P < .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P > .05).

Conclusions: Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I collagen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / enzymology*
  • Child
  • Child, Preschool
  • Collagen Type I / metabolism*
  • Dental Caries / complications
  • Dental Caries / microbiology*
  • Dentin / microbiology
  • Female
  • HIV Infections / complications*
  • Humans
  • Male
  • Mouth Mucosa / microbiology*
  • Proteolysis
  • Statistics, Nonparametric

Substances

  • Collagen Type I