Involvement of two-component system CBO0366/CBO0365 in the cold shock response and growth of group I (proteolytic) Clostridium botulinum ATCC 3502 at low temperatures

Miia Lindström; Elias Dahlsten; Henna Söderholm; Katja Selby; Panu Somervuo; John T Heap; Nigel P Minton; Hannu Korkeala

doi:10.1128/AEM.00555-12

Involvement of two-component system CBO0366/CBO0365 in the cold shock response and growth of group I (proteolytic) Clostridium botulinum ATCC 3502 at low temperatures

Appl Environ Microbiol. 2012 Aug;78(15):5466-70. doi: 10.1128/AEM.00555-12. Epub 2012 Jun 1.

Authors

Miia Lindström¹, Elias Dahlsten, Henna Söderholm, Katja Selby, Panu Somervuo, John T Heap, Nigel P Minton, Hannu Korkeala

Affiliation

¹ Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland. miia.lindstrom@helsinki.fi

Abstract

The role of the two-component system (TCS) CBO0366/CBO0365 in the cold shock response and growth of the mesophilic Clostridium botulinum ATCC 3502 at 15°C was demonstrated by induced expression of the TCS genes upon cold shock and impaired growth of the TCS mutants at 15°C.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acclimatization / physiology*
Bacterial Proteins / metabolism*
Clostridium botulinum / growth & development*
Cold Temperature*
Cold-Shock Response / physiology*
DNA Primers / genetics
Histidine Kinase
Phosphorylation
Protein Kinases / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / physiology*

Substances

Bacterial Proteins
DNA Primers
Protein Kinases
Histidine Kinase