Chronic unpredictable mild stress affects myocardial metabolic profiling of SD rats

J Pharm Biomed Anal. 2012 Nov:70:534-8. doi: 10.1016/j.jpba.2012.04.032. Epub 2012 May 10.

Abstract

Depression is frequently comorbid with cardiovascular diseases (CVDs), but a full understanding of the mechanisms is still on its way. Chronic unpredictable mild stress (CUMS) model is a commonly used model to mimic clinical depression, here we present a GC/MS-based metabolic profiling approach to investigate myocardial metabolic changes of CUMS SD rats. Principal Component Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) were utilized to reveal differences between the model and control group. This study found that molecules proved cardioprotective involving glutamine (P=0.019) and inosine (P=0.013), fatty acid (9,12-octadecadienoic acid, P=0.002; hexadecanoic acid, P=0.006; octadecanoic acid, P=0.030) which serve as the major energy source of heart and collagen molecule precursor proline (P=0.036) had down-regulated in CUMS model group.

MeSH terms

  • Animals
  • Behavior, Animal
  • Biomarkers / metabolism
  • Body Weight
  • Cardiovascular Diseases / etiology
  • Cardiovascular Diseases / metabolism*
  • Cardiovascular Diseases / prevention & control
  • Chronic Disease
  • Collagen / metabolism
  • Depression / complications
  • Depression / diagnosis
  • Depression / metabolism*
  • Depression / psychology
  • Disease Models, Animal
  • Energy Metabolism
  • Gas Chromatography-Mass Spectrometry
  • Least-Squares Analysis
  • Male
  • Metabolomics* / methods
  • Myocardium / metabolism*
  • Principal Component Analysis
  • Rats
  • Rats, Sprague-Dawley
  • Severity of Illness Index
  • Stress, Psychological / complications
  • Stress, Psychological / diagnosis
  • Stress, Psychological / metabolism*
  • Stress, Psychological / psychology

Substances

  • Biomarkers
  • Collagen