The J-aggregate of diprotonated tetraphenylporphyrin (H(4)TPP(2+)) formed at the dodecane-water interface showed circular dichroism spectra corresponding to the chirality of 2-alkyl alcohols, longer than 2-butanol, added to the dodecane phase. The phenomenon suggested the preferential interaction between the nano-sized J-aggregates and the chiral alcohols at the interface, and provided a potential use of the J-nanoaggregate as a chiral recognition probe.