Abstract
A wound biofilm model was created by adapting a superficial infection model. Partial-thickness murine wounds were inoculated with methicillin-resistant Staphylococcus aureus (MRSA). Dense biofilm communities developed at the wound surface after 24 h as demonstrated by microscopy and quantitative microbiology. Common topical antimicrobial agents had reduced efficacy when treatment was initiated 24 h after inoculation compared to 4 h after inoculation. This model provides a rapid in vivo test for new agents to treat wound biofilm infections.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Topical
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Animals
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Anti-Infective Agents, Local / administration & dosage*
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Anti-Infective Agents, Local / pharmacology
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Bacitracin / administration & dosage
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Bacitracin / pharmacology
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Biofilms / drug effects*
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Methicillin-Resistant Staphylococcus aureus / drug effects*
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Methicillin-Resistant Staphylococcus aureus / physiology
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Mice
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Mupirocin / administration & dosage
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Mupirocin / pharmacology
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Silver Compounds / administration & dosage
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Silver Compounds / pharmacology
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Silver Sulfadiazine / administration & dosage
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Silver Sulfadiazine / pharmacology
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Wound Infection / drug therapy
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Wound Infection / microbiology*
Substances
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Anti-Infective Agents, Local
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Silver Compounds
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Bacitracin
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Mupirocin
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Silver Sulfadiazine