Complete genome sequence of narcissus late season yellows virus infecting Chinese narcissus in China

Arch Virol. 2012 Sep;157(9):1821-4. doi: 10.1007/s00705-012-1328-5. Epub 2012 May 24.

Abstract

The complete genome sequence of a Chinese narcissus isolate of narcissus late season yellows virus from Zhangzhou, China (NLSYV-ZZ), was determined to be 9,651 nucleotides in length, excluding the 3'-terminal poly (A) tail, by amplification and sequencing of virus RNA. The viral genome contains a single long open reading frame of 9,315 nucleotides encoding a polyprotein of 3,105 amino acids. The polyprotein was predicted to be cleaved into ten mature proteins by three viral proteases. Complete genome sequence comparison and phylogenetic analysis indicated that NLSYV-ZZ was most closely related to narcissus yellow stripe virus (NYSV), which was also isolated from narcissus. These viruses shared 69.9 % identity in their complete nucleotide sequences and 77.0 % identity in their polyprotein amino acid sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • China
  • Cluster Analysis
  • Genome, Viral*
  • Molecular Sequence Data
  • Narcissus / virology*
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / virology*
  • Polyproteins / genetics
  • Potyvirus / genetics*
  • Potyvirus / isolation & purification
  • RNA, Viral / genetics*
  • Sequence Analysis, DNA*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Viral Proteins / genetics

Substances

  • Polyproteins
  • RNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/JQ326210